Silver In-situ Hybridization

The Concordance Between Immunohistochemical Staining And ...
Immunohistochemical (IHC) staining and silver in situ hybridization (SISH) for the HER2 status in breast cancer tissue samples exists. At Sundsvall Hospital, after performing IHC staining the positive samples are sent to a different laboratory for verification by gene amplification measurements with fluorescence in situ hybridization (FISH). ... Fetch Full Source

Targeting FGFR With Dovitinib (TKI258): Preclinical And ...
Amplification and hormone receptor (HR) status. FGFR1 amplification was assessed by silver in situ hybridization. Preplanned retrospective analyses assessed predictive value of FGFR1, FGFR2, and FGF3 amplifications by quantitative PCR (qPCR). Results: Dovitinib monotherapy inhibits proliferation in FGFR1- and FGFR2-amplified, but not FGFR- ... Return Doc

Distribution Of RRNA Pig hybridization silver-staining
An attempt was made to quantify the amount of silver deposited on the NORs by means of image analysis. The area of the silver deposit was divided by the length of the chromosome. RESULTS AND DISCUSSION In situ hybridization revealed that the secondary constrictions of both chromosomes ... Fetch Content

Genomic Alterations In Pulmonary Adenocarcinoma In Situ In An ...
Mutation Testing, Fluorescence In Situ Hybridization, and Silver In Situ Hybridization Mutation testing for EGFR was performed on DNA obtained from needle-microdissected tumor tissue from paraffin sections and analyzed by Sanger sequencing of exons 18 to 21. Mutation testing for KRAS was performed using similarly obtained DNA ... Access Doc

Molecular Testing Of Solid Tumors
In situ hybridization and silver in situ hybridization have become available as fully automated ERBB2 (HER2/neu) commercial kits and are approved for clinical use in Europe. These assays combine the benefits of IHC (low cost, use of a standard bright-field microscope with a light-stable reporter) and FISH (examination of genomic ... Access Full Source

Chromosome 7 Aneuploidy In Clear Cell And Papillary Renal ...
Fluorescent in situ hybridization and chromogenic in situ hybridization but not silver in situ hybridization (SISH). Aim: The purpose was to report chromosome 7 aberrations in ccRCC and pRCC using SISH in paraffin-embedded tissues and determine the association between the anomalies with clinical and pathological features. ... View Document

MM07-A2:Fluorescence In Situ Hybridization Methods For ...
This guideline primarily addresses “fluorescence” in situ hybridization because fluorescence is currently the most widely used method for demonstrating the location of the hybridized probe. Chromogenic in situ hybridization (CISH), silver precipitation in situ hybridization (SISH), and other nonfluorescent approaches are also in use. ... Get Document

VENTANA INFORM HER2 Dual ISH Assay - Reagent-catalog.roche.com
UltraView Silver ISH DNP Detection Kit (100 tests) ultraView Red ISH DIG Detection Kit (100 tests) 780-4422 760-098 760-505 Method Dual color in situ hybridization Storage 2–8°C Automation For use on BenchMark XT platform DNP HER2 DNP-labeled probe cohybridization A. INFORM HER2 Dual ISH DNA Probe Cocktail 1: Rabbit anti-DNP 2: Goat anti ... Access Doc

Computer-aided Software To Detect Breast Tumor Using In Situ ...
In situ hybridization techniques (ISH), whereas section 3 deals with the HER2 gene status. Section 4 presents the developed algorithm to estimate HER2 gene status in images treated with in situ hybridization techniques, together with the obtained results, and finally, section 5 presents the main conclusions and the future work. ... Access Doc

Germanium Dioxide - Wikipedia
Germanium dioxide, also called germanium oxide, germania, and salt of germanium, is an inorganic compound with the chemical formula Ge O 2. It is the main commercial source of germanium. It is the main commercial source of germanium. ... Read Article

Heat Shock Protein 90 (HSP90) And Her2 In Adenocarcinomas Of ...
2.3. In Situ Hybridization and Definition of Her2 Status Data for Her2 amplification were obtained from fluorescence in situ hybridization (FISH) or silver in situ hybridization (SISH) analysis [3,21]. A positive Her2 status was defined as immunohistochemical ... Document Viewer

In Situ hybridization - University Of Michigan
In situ hybridization, as the name suggests, is a method of localizing and detecting specific mRNA sequences in morphologically preserved tissues sections or cell preparations by hybridizing the complementary strand of a nucleotide probe to the sequence of interest. ... Read Here

H19 (gene) - Wikipedia
H19 is a gene for a long noncoding RNA, ("BWS"), as well as Silver-Russell syndrome. Gene characterization. The H19 gene contains 3 Sp1 binding sites, however In situ hybridization of the H19 RNA revealed that it localizes in a cytoplasmic ribonucleoprotein particle, ... Read Article

Zon Lab Overview - YouTube
Leonard Zon, MD, Director of the Steam Cell Research Program at Boston Children's Hospital, discusses how to increase blood stem cells by adding a chemical to cord blood. In Situ Hybridization ... View Video

Diagnostic Pathology BioMed Central
Metallography or silver in situ hybridization (SISH) [25-27]. The GOLDFISH procedure utilizes the tyramide sig-nal amplification principle and produces large clusters for amplified HER2 gene signal. On the other hand, the SISH method produces discrete metallic silver black signals. Horseradish peroxidase (HRP) of the detection system ... Retrieve Content

<Emphasis Type='Italic'>In Situ </Emphasis> hybridization ...
In situ hybridization technique using an immuno- gold silver staining system Summary An in situ hybridization technique using an immunogold silver staining detection system was used to detect biotiny- lated DNA probes in cultured cells and in formalin-fixed paraffin-embedded tissue. The detection method is rapid, ... Fetch Document

Improved Method Of Detecting The ERG Gene Rearrangement In ...
To detect this alteration, fluorescence in situ hybridization (FISH) is the method of choice. How-ever, FISH has some disadvantages for widespread adoption in clinical practice. Subsequently, chromo-genic in situ hybridization, which uses organic chro-mogens, and enzymatic metallography silver in situ hybridization have emerged as promising ... Retrieve Doc

Amplification Of The in Situ hybridization Signal By silver ...
Nal gland of the rat were hybridized in situ using a bio- tinylated oligonucleotide probe specific for tyrosine hy- droxylase (TH) messenger ribonucleic acid (mRNA). Hybridization was detected using the streptavidin-perox- idase-diaminobenzidine (DAB) system in combination with silver-gold postintensification. ... View Doc

Sanjeevni Hearb Fish Test For Coma(3) - YouTube
We put the silver shark fish 3 inches out of water for 20 min. Then put it into sanjeevni hearb water and fish was nirmal in 15 min. ... View Video

Interpretation Guide - Food And Drug Administration
UltraView SISH DNP (silver in situ hybridization) Detection Kit section (Figure 7). Cases representing the dynamic range of The Chrl7 centromere is targeted with a digoxigenin (DIG) HER2 status are shown in Figures 2-4. ... Fetch Content

Copy Number Variation In Breast Cancer Translational Research
Currently, fluorescence in situ hybridization (FISH) and silver in situ hybridization (SISH) are routine methodologies used to assess prognosis and drug eligibility. However, disadvantages with these methods include lengthy and laborious processes for staining and scoring slides, specialized training required for ... Access Full Source